ESR1 and melanoma: In melanoma cell line-A375, we found that an estrogen receptor antagonist, Y-134 was among the top 20 drugs which had the most similar (ranked by normalized connectivity score, FDR<0.05) induced gene expression pattern as the DE genes described above (Figure 4A, Supplementary Table 8), and an estrogen agonist, DY-131 had negative connectivity score (FDR<0.05), which indicates the induced gene expression pattern of this drug was opposing to our input DE gene expression profile (Supplementary Table 8).