Furthermore, two main indicators, P16 and P53, were characterized by immunohistochemical staining in adipose tissue which showed that the protein level of p16 and P53 in the T2DM + PTPN2 group after PTPN2 intervention was significantly decreased compared with the T2DM group (Figures 5L, M). Here, TP53 is linked to type 2 diabetes mellitus.