The results showed that chemogenetic activation of the oxytocin neurons in the PVN by CNO inflammatory pain‐induced anxiety‐like behaviors as measured by the EPM, light–dark transition, and marble burying tests (Figure 3D: mCherry:14.77 ± 3.114 s, hM3Dq: 51.24 ± 13.26 s, t = 3.158, p = 0.0049; Figure 3E: mCherry:0.1542 ± 0.03192, hM3Dq:0.2863 ± 0.05441, t = 2.240, p = 0.0379; Figure 3F: mCherry: 91.01 ± 2.779 s, hM3Dq:124.1 ± 5.876 s, t = 5.432, p < 0.0001; and Figure 3G: mCherry: 16.87 ± 0.5152, hM3Dq:9.250 ± 1.887, t = 4.287, p = 0.0002; n = 9–13). The gene discussed is OXT; the disease is Anxiety.