To further confirm the role of PLEKHG5 in HCC, we analyzed PLEKHG5 mRNA expression in The Cancer Genome Atlas (TCGA) database, and the results revealed that the PLEKHG5 mRNA level was upregulated in HCC patients, and increased with the progression of HCC (Fig. 2I; Fig. S2B). This evidence concerns the gene PLEKHG5 and hepatocellular carcinoma.