Furthermore, we detected PLEKHG5 protein levels in 32 HCC patients by western blotting and found that approximately 65% of sample pairs showed significantly increased PLEKHG5 expression in tumor tissues compared with paired normal tissues, while PLEKHG5 was undetectable in most of the other samples (Fig. 2C–D; Fig. S2A). The gene discussed is PLEKHG5; the disease is hepatocellular carcinoma.