Interestingly, DisGeneNet categories associated with significantly enriched proteins on VPS35 KO lysosomes included ‘Down Syndrome’, ‘Presenile Dementia’, ‘Amyloidosis’, ‘Alzheimer Disease’, ‘Parkinson Disease’, amongst others, indicating that the proteomic changes observed in these cells correlate with established phenotypes of neurodegeneration (Fig. 3n, Supplementary Fig. 4k, Supplementary Data 4). This evidence concerns the gene VPS35 and Down syndrome.