The brain is a very soft tissue, and TPM2 loss has been shown to promote the colonization of primary patient-derived glioblastoma cells on soft substrates.30 Rigidity-sensing modules have been analyzed by immunoblotting in many transformed cell lines, including the human breast cancer cell line MDA-MB-23, the fibrosarcoma cell line HT1080, and the mouse lung carcinoma cell line LCC. This evidence concerns the gene TPM2 and breast carcinoma.