Here, we utilized 5xFAD transgenic mice, which overexpress human APP and presenilin 1 (PSEN1) and harbor five familial AD (FAD) mutations: Swedish (K670N, M671L), Florida (I716V), and London (V717I) APP mutations as well as two mutations in PSEN1 (M146L and L286V) [37]. Here, PSEN1 is linked to familial Alzheimer disease.