We found that primary tumor cells with increased accessibility at the COL20A1 enhancer had enrichment for recognition motifs for proneural transcription factors such as ASCL1, NEUROD1, and NEUROG2 (Fig. 5f), whereas cells with decreased accessibility at the COL20A1 enhancer had depletion of DNA binding motifs associated with AP-1 family transcription factors such as FOS and JUND (Fig. 5g). Here, ASCL1 is linked to neoplasm.