CD8A and neoplasm: Strikingly, gene set enrichment analysis (GSEA) comparing CD8 + T cells between RMS subtypes indicated that dysfunction was more prevalent in FP samples, which were enriched for gene sets related to PD-1 signalling, oxidative phosphorylation and T cell exhaustion, while cells from FN tumours were enriched for interferon response and stimulation signatures (Fig. 2g).