It has been shown that the intratumoral, vaccine-induced, neoepitope-specific CD8+ T cells driving anti-tumor responses exhibit a less exhausted phenotype with lower expression of PD-1 and TIM-319 and that the tumor size negatively correlates with the frequency of intratumoral PD-1+ TIM-3+ CD8+ T cells in another preclinical cancer model46. The gene discussed is CD8A; the disease is neoplasm.