To assess the nevadensin-activated signaling pathways, KEGG pathway analysis of the above differentially expressed genes revealed the enrichment of the Hippo signaling system, the pluripotency of stem cells, p53 signaling pathway, ECM-receptor interaction, transcriptional misregulation in cancer, MAPK signaling pathway, Rap1 signaling pathway, proteoglycans in cancer, Apoptosis, PI3K-Akt signaling pathway, Calcium-signaling pathways, Cytokine-cytokine receptor interaction, cAMP signaling pathway, and Metabolic pathways (Fig. 3E). This evidence concerns the gene TP53 and cancer.