Cluster 2 comprised four genes, including Acta2, Col1a1, Col1a2, and Eln, genes that belong mainly to the extracellular matrix and morphogenesis processes and are involved in protein digestion and absorption, focal adhesion, proteoglycans in cancer, inflammatory response pathway, and dysregulated miRNAs targeting insulin/ PI3-AKT signaling. The gene discussed is COL1A1; the disease is cancer.