As curcumin possesses immunomodulatory properties and affects the NF-κB [31,32] and PD-1/PD-L1 [33] pathways, which are critical for T cell-mediated cell killing, we investigated the impacts caused by treatments on T-cell frequencies in the primary tumor draining lymph nodes (TDLN) and spleens in the tumor-bearing mice. This evidence concerns the gene CD274 and neoplasm.