More significantly, the resulting CLAN pM330/sgNtn1 successfully disrupted in vivo the expression of Ntn1 in macrophages and their Ntn1 gene in precursor monocytes, thereby reducing the expression of netrin-1 (encoded by Ntn1) and subsequently improving type 2 diabetes (T2D) symptoms. The gene discussed is NTN1; the disease is type 2 diabetes mellitus.