The strategy of using expressing plasmids also facilitates the clinical translation of proteins with high molecular weight, e.g., tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL), a potent anti-cancer molecule, which was transfected to MSCs for the production of TRAIL-enriched MSC-EVs displaying high cancer cell killing efficiency [102]. The gene discussed is TNFSF10; the disease is cancer.