Fluorescence microscopy and flow cytometry evidenced the higher uptake of DiI-labeled EXOs in NRP-1 positive human hepatoma cells, compared to NRP-1 negative cells; moreover, the combined effect of CUR and MFH induced significantly higher cytotoxicity than CUR-EXOs or plain SPIONs on both cell lines, and was more pronounced on the NRP-1 positive cells. The gene discussed is NRP1; the disease is hepatocellular carcinoma.