APJ ligand-induced protection against infarct size extension was also confirmed by studying biomarkers usually employed for MI diagnosing, such as creatinine kinase myocardial band (CK-MB) isoform, cardiac Troponin levels, and LDH release in plasma serum, which resulted as decreased by both native peptide administration [71,94,97]. This evidence concerns the gene APLNR and myocardial infarction.