The detection of high levels of ZIKV NS5 RNA 74 days post-infection in the one animal (O9355) examined by in situ hybridization confirms that the presence of NS1 detected by immunohistochemistry in the brains of all ZIKV-inoculated pups reflects persistent, active infections in the cerebellum at the time of euthanasia. The gene discussed is RAF1; the disease is infection.