In addition to proteins involved in EV biogenesis and release, such as tetraspanins (CD9, CD63, and CD81) and MVB-related proteins (ALIX and TSG101), proteins associated with cancer progression, including vascular endothelial growth factor (VEGF) and tumor necrosis factor-a (TNF-a), have also been identified by proteomics analyses [63,64]. Here, TNF is linked to cancer.