While basement-membrane-specific heparan sulfate proteoglycan core protein (HSPG2) and integrin beta-3 (ITGB3) (0.49 ± 0.18 fmol/μg) were measured in CRC cell line-derived EV samples, they were not detected in the WhL sample of the respective cell lines. Here, SDC2 is linked to colorectal carcinoma.