Non-competitive immunoassays for the multiplexed detection of three cytokines, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and interleukin-10 (IL-10), which are involved in cancer pathogenesis have also been developed using as labels Au nanoparticles that were firstly modified with Raman tags, covered with an Ag layer, and then the specific for each analyte antibodies were coupled [141]. This evidence concerns the gene TNF and cancer.