According to the results, the pre-treatment TF activity (AUC = 0.701, p = 0.0143) and PAI-1 (AUC = 0.659, p = 0.0472) concentration are considered to be the strongest predictors of disease relapse and may effectively predict breast cancer recurrence before the application of adjuvant therapy. The gene discussed is SERPINE1; the disease is breast cancer.