After verification of the successful 0.2 μm filtration by means of NTA (Figure 6B1, left), we showed that all three tetraspanins were basically detectable in the EVs, and as expected, CD63 signal was stronger in CD63-enriched (CD63+) EV fraction (Figure 6B1, right, data shown for EVs derived from mono-cultured PCa GBM cells). The gene discussed is CD63; the disease is glioblastoma.