In summary, we showed that both mono- and co-cultured TMZ+AT101/AT101 surviving GBM cells were characterized by the pronounced appearance of PS-positive GBM cells over time, exhibited an activation of the PI3K/Akt/mTOR pathway as well as a regulation of the GSK3β with subsequent changes in the expression of pro-tumorigenic genes, and, moreover, were characterized by changes in the amount and composition of EVs. Here, GSK3B is linked to glioblastoma.