Considering the structural relationship between the GPR18 and CB receptor system, as well as endoCB signaling control over emotions, food intake, pain sensation, and thermoregulation, we employed several screening tests assessing locomotor activity, depression-like and anxiety-like symptoms, pain threshold, core temperature, and food intake to study the effects of novel GPR18 ligands. The gene discussed is GPR18; the disease is depressive symptom measurement.