The metabolites depleted in SIRT2 KO mice, such as FAHFA (18:2/22:3), 6-aminopenicillanic acid, epinephrine, LPC (20:2), FAHFA (15:0/22:3), FAHFA (22:6/22:3), LPC (20:2(11Z,14Z)), and L-carnitine were negatively correlated with different NAFLD phenotypes. The gene discussed is SIRT2; the disease is metabolic dysfunction-associated steatotic liver disease.