Considering all these above results, we proposed an assumption that SIRT2 KO mice suffered from more serious gut microbiota dysbiosis and metabolic disorder induced by HFCS diet, which might account for the promotion of the NAFLD progression characterized by larger body weight and higher serum ALT, AST, TG, and CHO levels (Figure 9C). This evidence concerns the gene SIRT2 and metabolic dysfunction-associated steatotic liver disease.