Regarding the inhibition of NAFLD, metabolites (LPC and PC) in the downregulated choline metabolism and glycerophospholipid metabolism pathways positively correlated with a cluster of gut microbiota (Erysipelatoclostridium, Ileibacterium, Eggerthella, Atopobiaceae_unclassified, Enterococcus, Eubacterium, Erysipelatoclostridium_unclassified, and Blautia) which were downregulated in SIRT2 KO mice, and negatively correlated with two microbial genera (Monoglobus, Peptococcaceae_unclassified) which were upregulated in SIRT2 KO mice. The gene discussed is SIRT2; the disease is metabolic dysfunction-associated steatotic liver disease.