TP53 and Alzheimer disease: Additionally, the presence of iAβ, oxidative markers DJ-1 Cys106-SO3 and p-JUN, loss of ΔΨm, apoptosis markers TP53, PUMA, and CASP3, and dysfunctional ACh-induced Ca2+ influx were observed in mutant ChLNs and CSs in 11 days, whereas at least 35 days of culture are necessary to reproduce AD markers from mutant iPSCs::NPCs.