To measure the recombination efficiency of Hfe in KCs, we performed a liver perfusion in the HfeClec4fCre mutant and Hfefl/fl control mice, followed by differential centrifugation and magnetic cell sorting to obtain Kupffer cells (KC), liver sinusoidal endothelial cells (LSEC), hepatic stellate cells (HSC) and hepatocytes (HC). The gene discussed is HFE; the disease is dry eye syndrome.