GH1 and neoplasm: Clonogenic assay results, interestingly, showed notable differences in the number of colony-forming units in B16-F10 cells, as illustrated in Figure 2C. The quantitative histogram results demonstrated that 14 kDa hGH significantly reduced the number of colony-forming units in B16-F10 mixed clones and clone 8 cells compared to the control cells (Figure 2D), elucidating the tumor suppressor function of 14 kDa hGH.