Moreover, immunoblot data showed that among the top five E3 ubiquitin ligases (based on confidence scores in the UbiBrowser database), only NEDD4L could be upregulated by JAC4 treatment in human-lung-cancer cells (Figure 4C and Figure S5B), which was further confirmed in the JAC4-treated lung-cancer tumor-tissue samples (Figure 4D). The gene discussed is NEDD4L; the disease is lung cancer.