Additionally, due to the differences in the research results related to the effect of Ir on the progression of cancer in different types, the aim of this study was to examine the levels of EMT markers, such as E-cadherin, N-cadherin, SNAIL, SLUG, and TWIST, and to compare their expression levels with the level of FNDC5/Ir expression in BC. The gene discussed is TWIST1; the disease is breast cancer.