The ability of all the synthesized compounds to affect cell viability was studied by MTT assay using the human tumor cell lines HT-29 (colon adenocarcinoma), A-549 (pulmonary adenocarcinoma), and towards HEK-293 (human embryonic kidney cells), which does not express PD-L1 [25], human microvessel endothelial cells (HMEC-1) and Jurkat T cells. The gene discussed is CD274; the disease is colon adenocarcinoma.