To investigate the impact of Ezh2 in macrophages on hyper-inflammatory sepsis and sepsis after LPS tolerance, CLP after PBS injection (CLP) and CLP after twice LPS administration (LPS-CLP), respectively, in Ezh2 control (Ezhfl/fl; LysM-Cre−/−) and Ezh2 null (Ezhfl/fl; LysM-Cr cre/−) mice was performed (Figure 7A). Here, EZH2 is linked to Sepsis.