The former assessed the detection power of classical SjS-related antibodies (anti-Ro52, Ro60, and La) and distinguish 27 affected patients from 27 HC, and the latter explored the sensitivity and specificity of classical antigens (anti-Ro52, Ro60, and La) to identify subjects with sicca syndrome (20 subjects with SjS, 23 subjects with immune checkpoint inhibitor-induced sicca (ICIS), and 20 subjects with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED)) and to determine the detection power of salivary enriched proteins to define APECED subjects with sicca. This evidence concerns the gene RO60 and Sjogren syndrome.