To investigate the impact of the A429V mutation and the role of POC5 in the differentiation and function of primary osteoblasts derived from healthy donors without scoliosis and AIS patients, we performed alizarin red staining, alkaline phosphatase, and Western blot analyses of COL1A2 (Figure 2 and Figures S1–S3). This evidence concerns the gene COL1A2 and androgen insensitivity syndrome.