To investigate the impact of the A429V mutation and the role of POC5 in the differentiation and function of primary osteoblasts derived from healthy donors without scoliosis and AIS patients, we performed alizarin red staining, alkaline phosphatase, and Western blot analyses of COL1A2 (Figure 2 and Figures S1–S3). The gene discussed is POC5; the disease is androgen insensitivity syndrome.