To validate the above findings in vivo, we inoculated PC9, PC9/GR, PC9/GR DCLK1-KO (PC9/GR DCLK1−/− CTRL), and DCLK1-rescued PC9/GR cells (PC9/GR DCLK1−/− Res) subcutaneously in the abdomen of the mice, with 2 × 106 tumor cells per mouse. Here, DCLK1 is linked to neoplasm.