We found that VAS2870 ameliorated DSS-induced colitis in AMPKβ1LysM and reversed the LPS-induced TLR4 and NOX2 expression, ROS production, p65 NF-κB nuclear translocation, and proinflammatory cytokine secretion in bone marrow-derived macrophages of AMPKβ1fl/fl and AMPKβ1LysM mice. This evidence concerns the gene CYBB and colitis.