All rats were euthanized at week 22 after film application and histopathological evaluation of the tongue sections by light microscopy and immunohistochemical evaluation was performed to determine the effects of the material on the expression of the tumor and inflammatory markers Ki-67 (MIB-1), nuclear factor kappa B (NF-κB) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) [80]. This evidence concerns the gene MKI67 and neoplasm.