IRF6 and hypospadias: Among them, whole genome sequencing and variant annotation focused on genes known to be involved in CLJ and hypospadias in mammals identified 13 mutations with a high impact on the following overlapped genes: EGF, IRF6, BMPR1B, MMP14, ADGRA2, F13A1, CCDC8, ACVR1, BHMT2, CDH1, GSTP1, PHC2, and MYBPHL where the EGF, IRF6, F13A1, ADGRA2, and GSTP1 genes were found with homozygous mutation, and the CCDC8 and BMPR1B genes were not reported in the literature (except [30]) with implication in humans or animals CLJ or hypospadias, nor did the two genes in the Cleft Gene DataBase.