For example, C6-glioma, a widely used astroglial cell line derived from rat glioma, presents a significant defect, similar to other astrocytoma-derived cells, in the production of VPF (vascular permeability factor), which contributes to a great decrease in the barrier properties of the BBB in coculture [6]. Here, VEGFA is linked to astrocytoma (excluding glioblastoma).