In addition, several genes (ABCA2, CYP1A1, EPCAM, CCND1, CDC20, ITGA3, and MYC) that showed different expression profiles between 3D versus 2D cultures and GBM samples versus healthy brain samples in the GEO datasets were also replicated in the two larger cohorts. The gene discussed is ABCA2; the disease is glioblastoma.