When assessing LEP promoter methylation at the two time points using pyrosequencing, we found a significant increase in mean LEP promoter methylation at the time of progression from MDS to secondary AML (sAML) compared to the time of diagnosis with a relative increase in methylation of 20% in one patient and 92% in the other (Fig. 7). The gene discussed is LEP; the disease is myelodysplastic syndrome.