To identify a TMB-related genes’ signature of COAD, we conducted a various of bioinformatics analyses and identified a novel risk signature using ten TMB-related genes (GPRASP1, APLP1, ALPK3, SPTBN5, PCDHB14, LZTS3, RGL2, LINGO1, LZTS1 and CYP4F12). This evidence concerns the gene SPTBN5 and colon adenocarcinoma.