To determine the role of Trabid catalytic and ubiquitin chain-binding functions in tumor growth, we reconstituted Trabid knockdown cells with Trabid wild type or TrabidΔN/CS and found a higher promoting effect of Trabid wild type on tumor growth, comparing with TrabidΔN/CS (Fig. 6d, e and Supplementary Fig. 6b, c). This evidence concerns the gene ZRANB1 and neoplasm.