To determine the intrinsic versus acquired nature of dysregulated IFN signaling in CF MDMs, we carried out an experiment whereby patient MDMs were differentiated either in the presence or in the absence of CFTR-correcting Kaftrio, before LPS-activating the fully differentiated cells as before and assessing ISG expression by quantitative reverse transcription polymerase chain reaction (qRT-PCR). This evidence concerns the gene CFTR and cystic fibrosis.