Through analysis of potential biomarkers for differentially diagnosing ZIKV and DENV in endemic areas, our results suggest that IL-4 (p = 0.0176) could serve as a diagnostic target for distinguishing acute ZIKV from acute DENV infections, while IL-10 (p = 0.0003) could serve as a molecular marker for differentiating acute DENV from acute ZIKV infections, attributed to significant differences in the cytokine levels between the patient groups. The gene discussed is IL4; the disease is Zika virus infectious disease.