Experimentally in vivo and in vitro, PFOA and other well-tested PFASs cause visible evidence of grossly enlarged liver, biomarkers of liver injury such as higher ALT, oxidative stress, apoptosis hepatocyte proliferation and increased liver weight, triglyceride build-up as well as lipid/lipid droplet accumulation and ballooning of hepatocytes, along with other evidence of steatosis, and metabolic evidence of disrupted hepatic bile acid metabolism and adipogenesis, and these effects are seen across animal species and in vitro [8,9,13,20,23,55,56,57,58,59,60,61,62]. The gene discussed is GPT; the disease is steatosis.