The results of TNM staging, T staging, N staging, lymph node metastasis (LNM), tumor in the gallbladder neck, non-extended surgical range, vascular and nerve invasion, high level of FIB, fibrinogen/albumin (TRFA), carbohydrate antigen 125 (CA125), carbohydrate antigen 199 (CA199), glutamic oxalacetic transaminase (AST), FER, direct bilirubin (DBIL), total bile acid (TBA), ALP, gamma-glutamyl transpeptidase (GGT), total bilirubin (TBIL), neutrophil, and thrombocytocrit (PCT), a total of 22 clinical factors are risk factors for recurrence of GBC (Table S4). The gene discussed is ALB; the disease is metastatic malignant neoplasm in the lymph nodes.