In this context, the reduction of the expression of PI3K in the liver of adult animals with neonatal overnutrition observed in the present work is according to impairment of hepatic PI3K pathway in neonatal overfed animals in adulthood shown in previous studies (Conceição et al., 2013; Huang et al., 2020), despite the other molecules of this pathway was unchanged in the current data, suggesting a partial disruption of the insulin signaling pathway in the liver of adult male rats with neonatal overnutrition. This evidence concerns the gene INS and overnutrition.