The great potential of biopsy in cHCC-CCA diagnostication has also been highlighted in a recent study by Calderaro et al. which demonstrated that nestin, a progenitor cell marker, has strong diagnostic discriminatory power to distinguish cHCC-CCA from HCC (AUC 0.85, sensitivity 0.75, specificity 0.93). The gene discussed is NES; the disease is cholangiocarcinoma.