Scoring the severity of inflammation on a zero to ten scale and measuring the edema formation (ankle thickness and hind paw volume) revealed that lacking ARHGAP25 mitigated the symptoms of arthritis by about half (at days 3, 6, and 8, change in case of ankle thickening was 46%, 49%, and 53%, in the case of clinical score 35%, 56%, and 58%, and in the case of paw volume 100%, 63% and 64% respectively) compared to WT and this difference was significant (Figures 1B–D). This evidence concerns the gene ARHGAP25 and arthritic joint disease.